文章基本信息

标题：Optimisation of microwave-assisted extraction of flavonoids and phenolics from celery ( Apium graveolens L.) leaves by response surface methodology
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作者：He Q. ; Li Y. ; Zhang P. 等
期刊名称：Czech Journal of Food Sciences
印刷版ISSN：1212-1800
电子版ISSN：1805-9317
出版年度：2016
卷号：34
期号：4
页码：341-349
DOI：10.17221/266/2015-CJFS
出版社：Czech Academy of Agricultural Sciences
摘要：The conditions for microwave-assisted extraction (MAE) of total flavonoids (TF) and total phenols (TP), and antioxidant activity from celery (Apium graveolens L.) leaves by response surface methodology (RSM) were optimised. The 3-level, 3-factorial Box-Behnken design (BBD) was employed to study three main extraction conditions: microwave power (300–500 W), solid-solvent ratio (15–30 ml/g), and ethanol concentration (50–80%). It was found that microwave power of 500 W at 30 ml/g solid-solvent ratio with 75.6% (v/v) ethanol concentration was the most optimum conditions for the extraction of TF and TP from celery leaves with the consequent high antioxidant activity measured by the DPPH inhibition rate. Using the optimum extraction conditions, the extraction yields of TF and TP were 0.62 g RUE/100 g DW, 3.01 g GAE/100 g DW, respectively, and the DPPH inhibition rate was 88%. The results indicated that the nutritional quality of celery (Apium graveolens L.) leaves could be improved significantly by optimising the extraction process of MAE using response surface methodology.
关键词：Box-Behnken experimental design; total flavonoids; total phenolics; antioxidant activity