文章基本信息

标题：Influence of Different Solvents in the Recovery and Pre-Purification of Pectinases from Aspergillus niger
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作者：Marcia M. Santin Trentini ; Ana Paula Rossetto ; Ilizandra Aparecida Fernandes 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2018
卷号：14
期号：3
页码：69-76
DOI：10.19026/ajfst.14.5840
出版社：MAXWELL Science Publication
摘要：The present work aimed at studying the influence of different solvents (n-propyl alcohol, isopropyl alcohol and acetone) in the recovery and pre-purification of pectinases (exo-polygalacturonase-exo-PG, pectinmethylesterase-PME and pectin lyase-PMGL) from Aspergillus niger . The best results were obtained using isopropyl alcohol in the precipitated phase with purification factor and recovery of 2.1-fold and 20.5% for exo-PG, 7.9-fold and 77.8% for PME and 2.5-fold and 216% for PMGL enzyme. The studied method is promising in the recovery and pre-purification of pectinases enzymes.