文章基本信息

标题：Analytical Method Validation for Quantitative Estimation of Chloropicrin by HPLC Method
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作者：R. Swathi ; K.Aruna ; N.Venkatasubba Naidu 等
期刊名称：International Journal of Innovative Research in Science, Engineering and Technology
印刷版ISSN：2347-6710
电子版ISSN：2319-8753
出版年度：2017
卷号：6
期号：5
页码：8565
DOI：10.15680/IJIRSET.2017.0605209
出版社：S&S Publications
摘要：A simple, selective, precise and accurate High Performance Liquid Chromatographic method for theanalysis of Chloropicrin in its formulations was developed and validated in the present study. The mobile phaseconsists of a mixture of acetonitrile and water in the proportion 50: 50 (v/v). This was found to give sharp peak ofChloropicrin at a run time of 10 min. HPLC analysis of Chloropicrin was carried out at a wave length of 220 nm with aflow rate of 1.0mL/ min. The linear regression analysis data for the calibration curve showed a good linear relationshipwith a regression coefficient 0.999 in the concentration range of 50% to 150%. The linear regression equation was y=2055 x – 109.6. The developed method was employed with a high degree of precision and accuracy for the analysis ofChloropicrin. The method was validated for accuracy, precision, robustness, ruggedness and specificity. The Precision,accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is useful forthe quantification of Chloropicrin.
关键词：Chloropicrin; HPLC Method Development and Validation.