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标题：α-Chaconine and α-Solanine Inhibit RL95-2 Endometrium Cancer Cell Proliferation by Reducing Expression of Akt (Ser473) and ERα (Ser167)
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作者：Ayşe Kübra Karaboğa Arslan ; Mükerrem Betül Yerer
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2018
卷号：10
期号：6
页码：672
DOI：10.3390/nu10060672
语种：English
出版社：MDPI Publishing
摘要：The aim of this study is to investigate the potential inhibitory effect of α-chaconine and α-solanine on RL95-2 estrogen receptor (ER) positive human endometrial cancer cell line and to identify the effect of these glycoalkaloids on the Akt signaling and ERα. The cell proliferation profiles and the cytotoxicity studies were performed by Real-Time Cell Analyzer (xCELLigence) and compared with Sulphorhodamine B (SRB) assay. The effects of α-chaconine (2.5, 5, 10 µM), α-solanine (20, 30, 50 µM), API-1 (25 µM) and MPP (20 µM) effects on Akt (Ser473) and ERα (Ser167) expressions evaluated by Western blot and qPCR method. Their IC50 values were as α-chaconine (4.72 µM) < MPP (20.01 µM) < α-solanine (26.27 µM) < API-1 (56.67 µM). 10 μM α-chaconine and 20, 30 and 50 μM α-solanine were effective in decreasing p-Akt(Ser473)/Akt ratio compared to positive control API-1. When the p-ERα/ERα ratios were evaluated, it was observed that α-chaconine (2.5, 5, 10 μM) and α-solanine (50 μM) were as effective as the specific ERα inhibitor MPP in reducing the ratio of p-ERα/ERα compared to the control group. In conclusion, it has been shown that the proliferation of α-chaconine and α-solanine in human endometrial carcinoma cells reduces the expression and activity of the Akt and ERα signaling pathway.
关键词：α-chaconine; α-solanine; Solanum tuberosum L.; API-1; MPP dihydrochloride; RL95-2; endometrium cancer; steroidal glycoalkaloids; Akt; ERα α-chaconine ; α-solanine ; Solanum tuberosum L. ; API-1 ; MPP dihydrochloride ; RL95-2 ; endometrium cancer ; steroidal glycoalkaloids ; Akt ; ERα