文章基本信息

标题：The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis
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作者：Uun Yanuhar ; Achmad Khumaidi
期刊名称：Jurnal Akuakultur Indonesia
印刷版ISSN：1412-5269
出版年度：2017
卷号：16
期号：1
页码：22-32
DOI：10.19027/jai.16.1.22-32
语种：English
出版社：Jurnal Akuakultur Indonesia
其他摘要：ABSTRACT β-actin is a prominent protein in the immune system. An outcome from gene transcription, the protein protects against pathogen infection, such as receptor clustering, antigen internalization, and regulating vesicle for antigen processing. ß - actin expression will determine the success of immune response of the organism. This study aimed to understand the role of pigment-protein fraction (PPF) from Nannochloropsis oculata in the increase of β-actin expression in humpback grouper Cromileptes altivelis infected by viral nervous necrosis (VNN). The experiment was performed with one negative control (A: normal fish) and three PPF treatments (B: fish + PPF, C: fish + VNN, and D: fish + PPF + VNN). PPF was applied through the sonde method and analyzed with immunohistochemistry technique and Immunoratio software. The results showed that PPF was able to increase β-actin expression on all treatments: A (34.9%), B (38.1%), C (39.1%), and D (51.6%). It demonstrated PPF ability to induce the increase of β-actin expression indicating an improved defense of humpback grouper C. altivelis against VNN infection.