文章基本信息

标题：Dietary ambon lumut banana stem extract Musa cavendishii var. dwarf Paxton as an immunostimulant for white spot disease prevention in Pacific white shrimp Litopenaeus vannamei
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作者：Afriani Ramadhan ; Sri Nuryati ; Nur Bambang Priyoutomo 等
期刊名称：Jurnal Akuakultur Indonesia
印刷版ISSN：1412-5269
出版年度：2017
卷号：16
期号：2
页码：164-173
DOI：10.19027/jai.16.2.164-173
语种：English
出版社：Jurnal Akuakultur Indonesia
其他摘要：ABSTRACT This study was aimed to evaluate the dietary ambon lumut banana Musa cavendishii var. dwarf Paxton stem extract on the immune responses, growth, and survival rate of Pacific white shrimp against white spot disease. Ambon banana steam extract was obtained by maceration method using ethanol. The shrimps fed by pellet containing ambon banana stem extracts with different dosages for 29 days, i.e designated as 0.1 (A); 0.3 (B), and 0.5 (C) g/kg, the diet treatment without ambon banana stem extract without challenged test (K-), and diet treatment without ambon banana stem extract with challenged test (K+). Each treatment consisted of three replications. Feeding was conducted for 29 days of maintenance (four times a day). The results showed that the immune responses (average total hemocyte count: 45.15×10 6 cells/mL, phenoloxidase activity 1.03±0.08 OD, respiratory burst 0.95±0.04 OD, phagocytic activity 94.33±1.53%, growth (specific growth rate: 7.79±0.06%/day, feed ratio conversion was 52±0.01), and survival of treatment C (survival rate 100%) were higher compared with the treatment K+ (total hemocyte count: 3.83×10 6 cells/mL, phenoloxidase activity 0.04±0.01 OD, respiratory burst 0.18±0.06 OD, phagocytic activity 5.67±0.58%, specific growth rate: 2.61±0.08%/day, feed conversion ratio 2.11±0.02, survival rate: 50%). Therefore, banana stem extract at a dose of 0.5 g/kg everyday diet can be used to improve growth and nonspecific immune system against white spot disease on Pacific white shrimp.