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标题：Meniscus matrix morphological composition: age-dependent evaluation in a swine model
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作者：Umberto Polito ; Elena Dell'Era ; Irene Tessaro 等
期刊名称：International Journal of Health, Animal Science and Food Safety
印刷版ISSN：2283-3927
出版年度：2017
卷号：4
期号：1s
语种：English
出版社：Department of Health, Animal Science and Food Safety (VESPA)
摘要：Menisci are fibro-cartilaginous structures interposed between femoral condyle and tibial plateau, which have multiple functions in the stifle joint: act as shock absorbers, bear loaders and allow joint stability, congruity and lubrication (Sweigart et al ., 2004; Proffen et al ., 2012). It is well known that meniscal injuries lead to osteoarthritis and for these reasons, menisci are considered important target of investigation. Their important role in the knee wellness is only equalled by their deficiency in proper self-repairing. Nowadays, the gold standard technique is not just to remove the damaged meniscus, but to rebuild it or to replace it. For these reasons, studies are necessary to increase the knowledge about these small but essential structures (Streuli, 1999; Deponti et al ., 2013). Composition and morphology are basic fundamental information for the development of engineered meniscal substitutes (Di Giancamillo et al . , 2014). The analysis of the morphological, structural and biochemical changes, which occur during growth of the normal menisci, represent the goal of the present study. For this purpose, menisci from adult (7-month old), young (1-month old), and neonates (stillbirths) pigs were collected. Cellularity and glycosamiglycans (GAGs) deposition were evaluated by ELISA, while Collagen-1 and Collagen-2 were investigated by immunohistochemistry and Western blot analyses. Cellularity (P<0.01, all comparisons) and Collagen-1 (P<0.05, neonatal-young vs adult) decreased from neonatal to adult stage while GAGs (P<0.01 neonatal vs young-adult) and Collagen-2 (P<0.01 neonatal-young vs adult) showed the opposite trend. Immunohistochemistry revealed similar changes occurring during animal growth thus revealing that cellular phenotype, cellularity and protein expression, as well as fibers aggregation in the matrix, are dissimilar in the three ages analysed categories. These changes reflect the progressive menisci maturation and hyper-specialisation. We observed the correlation between biochemical and phenotype properties of swine menisci follow age-dependent changes during growth: starting with an immature cellular and fiber pattern to the mature organised and differentiated adult menisci. Acknowledgments: This work was funded by the “Finanziamento Piano Sviluppo Ateneo - Linea 2A”