文章基本信息

标题：Callus Induction and Somatic Embryogenesis from Cultured Zygotic Embryo of Eleiodoxa conferta (Griff.) Burr., an Edible Native Plant Species in Southern Thailand
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作者：Duangkhaetita KANJANASOPA ; Benjamas SOMWONG ; Theera SRISAWAT 等
期刊名称：Walailak Journal of Science and Technology (WJST)
印刷版ISSN：2228-835X
出版年度：2016
卷号：14
期号：11
页码：875-882
语种：English
出版社：Institute of Research and Development, Walailak University.
摘要：This research aimed to study the in vitro culturing of Eleiodoxa conferta (Griff.) Burr., collected from the Natural Study Center of Khan Thuli Peat Swamp Forest, located at Amphor Tha Chana, Surat Thani province. Primarily, the explant types for callus induction were investigated, and it was found that zygotic embryo is a suitable explant source, with a high potential of responsive tissue, and lacking browning secretions during culturing. The callus induction process was investigated by culturing zygotic embryos on MS medium supplemented with dicamba concentrations of 1.0, 2.5, and 5.0 mg.L -1 combined with 200 mg.L -1 ascorbic acid. The best 74.16 % callus response was obtained with 2.5 mg.L -1 dicamba. Callus proliferation was good on a medium with the reduced dicamba concentration of 0.5 mg.L -1 , giving the largest 0.41 cm callus size, and the highest 0.141 g callus fresh weight. Embryogenic callus competence was successfully induced at 38.33 % when culturing with 0.1 mg.L -1 dicamba and 1.0 g.L -1 casein hydrolysate. Embryogenic calli clump cultivated on MN6 medium developed to somatic embryos in the globular stage at 95.23 %. This suggests that the NO3/NO3+NH4 ratio and vitamin contents in MN6 could promote somatic embryogenesis.