文章基本信息

标题：Locus-specific EPIC-PCR primers for four distinct calmodulin genes of the Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844)
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作者：Seinen Chow ; Nobuaki Suzuki ; Yoji Nakamura 等
期刊名称：International Aquatic Research
印刷版ISSN：2008-6970
出版年度：2017
卷号：9
期号：1
页码：53-59
DOI：10.1007/s40071-017-0155-4
语种：English
出版社：BioMed Central
摘要：Nucleotide sequences of four distinct calmodulin genes (designated as CaM -A to -D) of the Pacific bluefin tuna ( Thunnus orientalis ) were compared. Nucleotide sequence divergence between the coding regions of four genes ranged from 16.0 to 21.6%, and locus-specific eight primer pairs targeting introns were designed. These primer pairs amplified expected size of fragments in five congeneric tuna species, and utility of these primer pairs was investigated using distant fish species.
关键词：Thunnus orientalis ; Four calmodulin genes ; Locus-specific primers ; Cross-species amplification ; DNA marker