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标题：Evaluation of anticancer activity of water and juice extracts of young Hordeum vulgare in human cancer cell lines HT-29 and A549
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作者：Arkadiusz Czerwonka ; Katarzyna Kawka ; Klaudia Cykier 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2017
卷号：24
期号：2
页码：345-349
DOI：10.26444/aaem/74714
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction and objective. Barley (Hordeum vulgare L.) is known as a rich source of different bioactive compounds. Atpresent, considerable attention of researchers is focused on young barley grass. It can be a good source of dietary minerals,vitamins, carbohydrates, amino acids, phenolic compounds and proteins. It is possible that the composition of chemicalingredients beneficial for health may induce an anticancer potential of young barley in human colon adenocarcinoma (HT-29) and human lung adenocarcinoma (A549) cell lines.Materials and method. Hordeum vulgare water extract (HWE) and Hordeum vulgare juice extract (HJE) were prepared.Cell proliferation and viability were examined with the use of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide) and NR (neutral red) methods. Induction of necrosis was assessed by propidium iodide/Hoechst staining. Progressof the cell cycle involved in cell proliferation, apoptosis, and regulation of transcription was estimated using flow cytometryanalysis. Additionally, the capability of free radical scavenging was evaluated with the DPPH assay.Results. The study revealed that extracts inhibited the proliferation of cancer cells. The NR study confirmed the low cytotoxicactivity of the tested extracts to normal human colon epithelial cells (CCD 841 CoTr) and human skin fibroblasts (HSF).Furthermore, a dose-dependent cytotoxicity against HT-29 cells, but not A549 cells, has been reported. The free radicalscavenging activity was observed in the case of the HWE but not the HJE.Conclusions. The obtained results indicate a cancer chemopreventive potential of young barley as a safe dietary agent incolon carcinoma.
关键词：Hordeum vulgare; colon cancer; lung cancer; inhibition of proliferation; necrosis