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标题：The peripheral quantitative computed tomographic and densitometric analysis of skeletal tissue in male Wistar rats after chromium sulfate treatment
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作者：Marek Bieńko ; Radosław Piotr Radzki ; Dariusz Wolski 等
期刊名称：Annals of Agricultural and Environmental Medicine
印刷版ISSN：1232-1966
电子版ISSN：1898-2263
出版年度：2017
卷号：24
期号：3
页码：446-452
DOI：10.26444/aaem/74585
出版社：Institute of Agricultural Medicine in Lublin
摘要：Introduction. This study evaluates the effects of three different doses of chromium sulphate on bone density and thetomographic parameters of skeletal tissue of rats.Materials and method. The experiment was performed on 40 male Wistar rats which received, by gavage, during 90 days,a chromium sulphate in either a daily dose of 400, 600 or 800 μg/kg BW. At the end of experiment, the rats were scannedusing the densitometry method (DXA) to determine the bone mineral density, bone mineral content of total skeleton andvertebral column (L2-L4) and parameters of body composition (Lean Mass and Fat Mass). The isolated femora were scannedusing peripheral a quantitative computed tomography method (pQCT) for a separate analysis of the trabecular and corticalbone tissue. The ultimate strength, work to ultimate and the Young modulus of femora was also investigated by the threepointbending test.Results. The negative impact of chromium was observed in relation to bone tissue. All doses significantly decreased totalskeleton density and mineral content, and also had impact upon the isolated femora and vertebral column. Trabecularvolumetric bone mineral density and trabecular bone mineral content measured by pQCT in distal femur metaphysis weresignificantly lower in the experimental groups than in the control. Higher doses of chromium also significantly decreasedvalues of ultimate strength and Young modulus in the investigated femora.Conclusions. The results of the experiment demonstrate that chromium sulphate is dose dependent, and exerts adisadvantageous effect on the skeleton, as it decreases bone density and resistance.
关键词：rat; chromium sulfate; bone; densitometry; peripheral quantitative computed tomography; bone mineral density and content