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标题：Characterization of non-typhoidal Salmonella enterica strains of human origin in central and southern Italy
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作者：Yolande T.R. Proroga ; Federico Capuano ; Rosanna Capparelli 等
期刊名称：Italian Journal of Food Safety
电子版ISSN：2239-7132
出版年度：2018
卷号：7
期号：1
DOI：10.4081/ijfs.2018.6888
语种：English
出版社：PAGEPress Publications
摘要：Non-typhoidal Salmonella enterica infection is a significant public health problem worldwide. The aim of this study was to characterize Salmonella enterica strains isolated from human specimens in central and southern Italy, for epidemiological studies. One hundred and fifty S. enterica strains were serotyped. Isolates were tested for their antimicrobial susceptibility, by disk diffusion method. The molecular characterizations, based on PCR, were carried out for the detection of invA gene and other virulence elements and phage marker genes. Eighteen different Salmonella serotypes were identified. The most common serotypes detected were S. Typhimurium, S . Enteritidis, the monophasic variant of S. Typhimurium ( S. 4,[5],12:i:-), and S . Napoli. High resistance rates were recorded for tetracycline (64%), streptomycin (62%), sulphonamide (57%), and ampicillin (56%). The ASSuT R-type, also associated to resistance to other antibiotics, was highly prevalent in S. 4,[5],12:i:- (97%) and S . Typhimurium (55%), while the ACSSuT Rtype, also associated to other antibiotics, was observed prevalently in S. Typhimurium (20.4%). The genes of more common detection were invA (100%), sspH2 (86.6%), gtgB (84.6%), g8 (80%), sodC1 (77.3%), gipA (52.6%), sspH1 (52.6%).
关键词：Salmonella enterica; human isolates; antibiotic resistance; R-type ASSuT; Rtype ACSSuT; virulotype