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标题：Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia
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作者：Hiten D. Mistry ; Lesia O. Kurlak ; Yosef T. Mansour 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2017
卷号：58
期号：6
页码：1186-1195
DOI：10.1194/jlr.M071985
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Preeclampsia is a pregnancy-specific condition that leads to increased cardiovascular risk in later life. A decrease in cholesterol efflux capacity is linked to CVD. We hypothesized that in preeclampsia there would be a disruption of maternal/fetal plasma to efflux cholesterol, as well as differences in the concentrations of both placental sterol 27-hydroxylase (CYP27A1) and apoA1 binding protein (AIBP). Total, HDL-, and ABCA1-mediated cholesterol effluxes were performed with maternal and fetal plasma from women with preeclampsia and normotensive controls (both n = 17). apoA1 and apoE were quantified by chemiluminescence, and 27-hydroxycholesterol (27-OHC) by GC-MS. Immunohistochemistry was used to determine placental expression/localization of CYP27A1, AIBP, apoA1, apoE, and SRB1. Maternal and fetal total and HDL-mediated cholesterol efflux capacities were increased in preeclampsia (by 10–20%), but ABCA1-mediated efflux was decreased (by 20–35%; P 0.05). Maternal and fetal apoE concentrations were higher in preeclampsia. Fetal plasma 27-OHC levels were decreased in preeclamptic samples ( P P = 0.04). Placental 27-OHC concentrations were also raised in preeclampsia ( P
关键词：lipid/efflux ; 27-hydroxycholesterol ; apolipoproteins ; pregnancy ; hypertension ; sterol 27-hydroxylase