文章基本信息

标题：The influence of placental metabolism on fatty acid transfer to the fetus
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作者：Simone Perazzolo ; Birgit Hirschmugl ; Christian Wadsack 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2017
卷号：58
期号：2
页码：443-454
DOI：10.1194/jlr.P072355
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：The factors determining fatty acid transfer across the placenta are not fully understood. This study used a combined experimental and computational modeling approach to explore placental transfer of nonesterified fatty acids and identify the rate-determining processes. Isolated perfused human placenta was used to study the uptake and transfer of ¹³C-fatty acids and the release of endogenous fatty acids. Only 6.2 ± 0.8% of the maternal ¹³C-fatty acids taken up by the placenta was delivered to the fetal circulation. Of the unlabeled fatty acids released from endogenous lipid pools, 78 ± 5% was recovered in the maternal circulation and 22 ± 5% in the fetal circulation. Computational modeling indicated that fatty acid metabolism was necessary to explain the discrepancy between uptake and delivery of ¹³C-fatty acids. Without metabolism, the model overpredicts the fetal delivery of ¹³C-fatty acids 15-fold. Metabolic rate was predicted to be the main determinant of uptake from the maternal circulation. The microvillous membrane had a greater fatty acid transport capacity than the basal membrane. This study suggests that incorporation of fatty acids into placental lipid pools may modulate their transfer to the fetus. Future work needs to focus on the factors regulating fatty acid incorporation into lipid pools.
关键词：placenta ; fatty acids ; placental transport ; dual placental perfusion ; lipid computational model ; compartmental modelling