文章基本信息

标题：Identification of roles for H264, H306, H439, and H635 in acid-dependent lipoprotein release by the LDL receptor
本地全文：下载
作者：Hongyun Dong ; Zhenze Zhao ; Drake G. LeBrun 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2017
卷号：58
期号：2
页码：364-374
DOI：10.1194/jlr.M070938
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Lipoproteins internalized by the LDL receptor (LDLR) are released from this receptor in endosomes through a process that involves acid-dependent conformational changes in the receptor ectodomain. How acidic pH promotes this release process is not well understood. Here, we assessed roles for six histidine residues for which either genetic or structural data suggested a possible role in the acid-responsiveness of the LDLR. Using assays that measured conformational change, acid-dependent lipoprotein release, LDLR recycling, and net lipoprotein uptake, we show that H635 plays important roles in acid-dependent conformational change and lipoprotein release, while H264, H306, and H439 play ancillary roles in the response of the LDLR to acidic pH.
关键词：dyslipidemias ; endocytosis ; low density lipoprotein ; lipoproteins/metabolism ; lipoproteins/receptors