文章基本信息

标题：Monoclonal antibodies that bind to the Ly6 domain of GPIHBP1 abolish the binding of LPL
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作者：Xuchen Hu ; Mark W. Sleeman ; Kazuya Miyashita 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2017
卷号：58
期号：1
页码：208-215
DOI：10.1194/jlr.M072462
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：GPIHBP1, an endothelial cell protein, binds LPL in the interstitial spaces and shuttles it to its site of action inside blood vessels. For years, studies of human GPIHBP1 have been hampered by an absence of useful antibodies. We reasoned that monoclonal antibodies (mAbs) against human GPIHBP1 would be useful for 1 ) defining the functional relevance of GPIHBP1’s Ly6 and acidic domains to the binding of LPL; 2 ) ascertaining whether human GPIHBP1 is expressed exclusively in capillary endothelial cells; and 3 ) testing whether GPIHBP1 is detectable in human plasma. Here, we report the development of a panel of human GPIHBP1-specific mAbs. Two mAbs against GPIHBP1’s Ly6 domain, RE3 and RG3, abolished LPL binding, whereas an antibody against the acidic domain, RF4, did not. Also, mAbs RE3 and RG3 bound with reduced affinity to a mutant GPIHBP1 containing an Ly6 domain mutation (W109S) that abolishes LPL binding. Immunohistochemistry studies with the GPIHBP1 mAbs revealed that human GPIHBP1 is expressed only in capillary endothelial cells. Finally, we created an ELISA that detects GPIHBP1 in human plasma. That ELISA should make it possible for clinical lipidologists to determine whether plasma GPIHBP1 levels are a useful biomarker of metabolic or vascular disease
关键词：chylomicrons ; dyslipidemias ; endothelial cells ; lipoprotein lipase ; triglycerides