文章基本信息

标题：Sweat lipid mediator profiling: a noninvasive approach for cutaneous research
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作者：Karan Agrawal ; Lauren A. Hassoun ; Negar Foolad 等
期刊名称：JLR Papers In Press
印刷版ISSN：0022-2275
电子版ISSN：1539-7262
出版年度：2017
卷号：58
期号：1
页码：188-195
DOI：10.1194/jlr.M071738
语种：English
出版社：American Society for Biochemistry and Molecular Biology
摘要：Recent advances in analytical and sweat collection techniques provide new opportunities to identify noninvasive biomarkers for the study of skin inflammation and repair. This study aims to characterize the lipid mediator profile including oxygenated lipids, endocannabinoids, and ceramides/sphingoid bases in sweat and identify differences in these profiles between sweat collected from nonlesional sites on the unflared volar forearm of subjects with and without atopic dermatitis (AD). Adapting routine procedures developed for plasma analysis, over 100 lipid mediators were profiled using LC-MS/MS and 58 lipid mediators were detected in sweat. Lipid mediator concentrations were not affected by sampling or storage conditions. Increases in concentrations of C30–C40 [NS] and [NdS] ceramides, and C18:1 sphingosine, were observed in the sweat of study participants with AD despite no differences being observed in transepidermal water loss between study groups, and this effect was strongest in men ( P
关键词：oxidized lipids ; endocannabinoids ; ceramides ; sphingolipids ; skin ; metabolic profiling ; non-invasive sampling ; atopic dermatitis