文章基本信息

标题：Atomic mutagenesis of stop codon nucleotides reveals the chemical prerequisites for release factor-mediated peptide release
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作者：Thomas Philipp Hoernes ; Nina Clementi ; Michael Andreas Juen 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2018
卷号：115
期号：3
页码：E382-E389
DOI：10.1073/pnas.1714554115
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Termination of protein synthesis is triggered by the recognition of a stop codon at the ribosomal A site and is mediated by class I release factors (RFs). Whereas in bacteria, RF1 and RF2 promote termination at UAA/UAG and UAA/UGA stop codons, respectively, eukaryotes only depend on one RF (eRF1) to initiate peptide release at all three stop codons. Based on several structural as well as biochemical studies, interactions between mRNA, tRNA, and rRNA have been proposed to be required for stop codon recognition. In this study, the influence of these interactions was investigated by using chemically modified stop codons. Single functional groups within stop codon nucleotides were substituted to weaken or completely eliminate specific interactions between the respective mRNA and RFs. Our findings provide detailed insight into the recognition mode of bacterial and eukaryotic RFs, thereby revealing the chemical groups of nucleotides that define the identity of stop codons and provide the means to discriminate against noncognate stop codons or UGG sense codons.
关键词：ribosome ; translation ; peptide release ; release factor ; mRNA modification