文章基本信息

标题：Linking secondary metabolites to gene clusters through genome sequencing of six diverse Aspergillus species
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作者：Inge Kjærbølling ; Tammi C. Vesth ; Jens C. Frisvad 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2018
卷号：115
期号：4
页码：E753-E761
DOI：10.1073/pnas.1715954115
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The fungal genus of Aspergillus is highly interesting, containing everything from industrial cell factories, model organisms, and human pathogens. In particular, this group has a prolific production of bioactive secondary metabolites (SMs). In this work, four diverse Aspergillus species ( A. campestris , A. novofumigatus , A. ochraceoroseus , and A. steynii ) have been whole-genome PacBio sequenced to provide genetic references in three Aspergillus sections. A. taichungensis and A. candidus also were sequenced for SM elucidation. Thirteen Aspergillus genomes were analyzed with comparative genomics to determine phylogeny and genetic diversity, showing that each presented genome contains 15–27% genes not found in other sequenced Aspergilli. In particular, A. novofumigatus was compared with the pathogenic species A. fumigatus . This suggests that A. novofumigatus can produce most of the same allergens, virulence, and pathogenicity factors as A. fumigatus , suggesting that A. novofumigatus could be as pathogenic as A. fumigatus . Furthermore, SMs were linked to gene clusters based on biological and chemical knowledge and analysis, genome sequences, and predictive algorithms. We thus identify putative SM clusters for aflatoxin, chlorflavonin, and ochrindol in A. ochraceoroseus , A. campestris , and A. steynii , respectively, and novofumigatonin, ent -cycloechinulin, and epi -aszonalenins in A. novofumigatus . Our study delivers six fungal genomes, showing the large diversity found in the Aspergillus genus; highlights the potential for discovery of beneficial or harmful SMs; and supports reports of A. novofumigatus pathogenicity. It also shows how biological, biochemical, and genomic information can be combined to identify genes involved in the biosynthesis of specific SMs.
关键词：Aspergillus ; fumigatus ; comparative genomics ; secondary metabolism