The Humor Styles Questionnaire (HSQ: Martin et al., 2003) is a self-report measure to evaluate individual differences in the use of humor styles. The present study investigated the reliability and validity of the Japanese version of the HSQ (HSQ-J: Yoshida, 2012). The results of confirmatory factor analysis and internal consistency demonstrated adequate fit for a four-factor structure with reliability for each factor. Validity assessment of the HSQ-J indicated that the four humor styles related significantly as predicted to measures of self-esteem, aggression, social skills, and Japanese-derived humor scales. Based on the results, additional cultural considerations were discussed.