文章基本信息

标题：Kinematics of flagellar swimming in Euglena gracilis: Helical trajectories and flagellar shapes
本地全文：下载
作者：Massimiliano Rossi ; Giancarlo Cicconofri ; Alfred Beran 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：50
页码：13085-13090
DOI：10.1073/pnas.1708064114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The flagellar swimming of euglenids, which are propelled by a single anterior flagellum, is characterized by a generalized helical motion. The 3D nature of this swimming motion, which lacks some of the symmetries enjoyed by more common model systems, and the complex flagellar beating shapes that power it make its quantitative description challenging. In this work, we provide a quantitative, 3D, highly resolved reconstruction of the swimming trajectories and flagellar shapes of specimens of Euglena gracilis . We achieved this task by using high-speed 2D image recordings taken with a conventional inverted microscope combined with a precise characterization of the helical motion of the cell body to lift the 2D data to 3D trajectories. The propulsion mechanism is discussed. Our results constitute a basis for future biophysical research on a relatively unexplored type of eukaryotic flagellar movement.
关键词：Euglena gracilis ; microswimmers ; microscopy imaging ; 3D flagellum shapes ; helical trajectories