文章基本信息

标题：Structural changes of tailless bacteriophage ΦX174 during penetration of bacterial cell walls
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作者：Yingyuan Sun ; Aaron P. Roznowski ; Joshua M. Tokuda 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：52
页码：13708-13713
DOI：10.1073/pnas.1716614114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Unlike tailed bacteriophages, which use a preformed tail for transporting their genomes into a host bacterium, the ssDNA bacteriophage ΦX174 is tailless. Using cryo-electron microscopy and time-resolved small-angle X-ray scattering, we show that lipopolysaccharides (LPS) form bilayers that interact with ΦX174 at an icosahedral fivefold vertex and induce single-stranded (ss) DNA genome ejection. The structures of ΦX174 complexed with LPS have been determined for the pre- and post-ssDNA ejection states. The ejection is initiated by the loss of the G protein spike that encounters the LPS, followed by conformational changes of two polypeptide loops on the major capsid F proteins. One of these loops mediates viral attachment, and the other participates in making the fivefold channel at the vertex contacting the LPS.
关键词：bacteriophage ; ΦX174 ; lipopolysaccharide ; cryo-EM ; DNA ejection