文章基本信息

标题：Synthesis of Galactosyl Mannitol Derivative Using β-Galactosidase from Kluyveromyces lactis
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作者：Robert Klewicki ; Iwona Belina ; Aleksandra Wojciechowska 等
期刊名称：Polish Journal of Food and Nutrition Sciences
印刷版ISSN：1230-0322
电子版ISSN：2083-6007
出版年度：2017
卷号：67
期号：1
页码：33-40
DOI：10.1515/pjfns-2016-0002
语种：English
出版社：Walter de Gruyter GmbH
摘要：The purpose of the study was to identify the influence of reactive mixture concentration (23–48 g/100 mL), pH (6.5–9.0), presence of NaCl (0.05–0.25 mol/L) and enzyme dose (2850–28,500 LAU/100 g of lactose) on the synthesis of galactosyl mannitol derivative using β-galactosidase from Kluyveromyces lactis . The use of the enzyme dose ranging from 2850 to 11,400 LAU/100 g lactose allowed obtaining gal-mannitol at the level of 21.8% total saccharides; higher doses intensified product decomposition. An increase in the concentration of the reactive mixture had a positive impact on the quantity of gal-mannitol obtained every single time, i.e. 4.39 g were obtained from 100 mL of a 23 g/100 mL solution and over 10 g were obtained from a 48 g/100 mL solution. A relatively low increase in product quantity (by ca . 5%) occurred after the pH was increased from 6.5 to 9.0. The use of NaCl rendered better results. An increase in the maximum content of gal-mannitol in the total sugar by 12.8% was observed at the concentration of 0.25 mol/L.
关键词：mannitol ; β-galactosidase ; transglycosylation ; lactose