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标题：Fructose-Rich Diet Affects Mitochondrial DNA Damage and Repair in Rats
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作者：Federica Cioffi ; Rosalba Senese ; Pasquale Lasala 等
期刊名称：Nutrients
电子版ISSN：2072-6643
出版年度：2017
卷号：9
期号：4
页码：323
DOI：10.3390/nu9040323
语种：English
出版社：MDPI Publishing
摘要：Evidence indicates that many forms of fructose-induced metabolic disturbance are associated with oxidative stress and mitochondrial dysfunction. Mitochondria are prominent targets of oxidative damage; however, it is not clear whether mitochondrial DNA (mtDNA) damage and/or its lack of repair are events involved in metabolic disease resulting from a fructose-rich diet. In the present study, we evaluated the degree of oxidative damage to liver mtDNA and its repair, in addition to the state of oxidative stress and antioxidant defense in the liver of rats fed a high-fructose diet. We used male rats feeding on a high-fructose or control diet for eight weeks. Our results showed an increase in mtDNA damage in the liver of rats fed a high-fructose diet and this damage, as evaluated by the expression of DNA polymerase γ, was not repaired; in addition, the mtDNA copy number was found to be significantly reduced. A reduction in the mtDNA copy number is indicative of impaired mitochondrial biogenesis, as is the finding of a reduction in the expression of genes involved in mitochondrial biogenesis. In conclusion, a fructose-rich diet leads to mitochondrial and mtDNA damage, which consequently may have a role in liver dysfunction and metabolic diseases.
关键词：fructose-rich diet; mitochondrial biogenesis; mitochondrial DNA (mtDNA); oxidative damage; repair mechanisms fructose-rich diet ; mitochondrial biogenesis ; mitochondrial DNA (mtDNA) ; oxidative damage ; repair mechanisms