文章基本信息

标题：Evaluation of the periodontal regenerative properties of patterned human periodontal ligament stem cell sheets
本地全文：下载
作者：Kim, Joong-Hyun ; Ko, Seok-Yeong ; Lee, Justin Ho 等
期刊名称：The Journal of the Korean Academy of Periodontology
印刷版ISSN：0250-3352
出版年度：2017
卷号：47
期号：6
页码：402-415
DOI：10.5051/jpis.2017.47.6.402
语种：English
出版社：Korean Academy of Periodontology
摘要：Purpose
The aim of this study was to determine the effects of patterned human periodontal ligament stem cell (hPDLSC) sheets fabricated using a thermoresponsive substratum.
Methods
In this study, we fabricated patterned hPDLSC sheets using nanotopographical cues to modulate the alignment of the cell sheet.
Results
The hPDLSCs showed rapid monolayer formation on various surface pattern widths. Compared to cell sheets grown on flat surfaces, there were no significant differences in cell attachment and growth on the nanopatterned substratum. However, the patterned hPDLSC sheets showed higher periodontal ligamentogenesis-related gene expression in early stages than the unpatterned cell sheets.
Conclusions
This experiment confirmed that patterned cell sheets provide flexibility in designing hPDLSC sheets, and that these stem cell sheets may be candidates for application in periodontal regenerative therapy.
Go to: Graphical Abstract
关键词：extracellular matrix; Periodontal ligament; Regeneration; Stem Cells; Tissue engineering