文章基本信息

标题：Solvent Optimization For Genistein Isolation Of “Rotten Tempe” By High Performance Liquid Chromatography Method
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作者：Cucun Alep Riyanto ; Hartati Soetjipto
期刊名称：Jurnal Eksakta
印刷版ISSN：1411-1047
电子版ISSN：2503-2364
出版年度：2017
卷号：17
期号：2
页码：111-118
DOI：10.20885/eksakta.vol17.iss2.art3
语种：English
出版社：Jurnal Eksakta
其他摘要：Genistein is a soy isoflavone that has been known have anticancer properties. The aim of this research is to determine the right combination of solvents to extract isoflavones in rotten tempe and determine the genistein content of the extraction process by the High Performance Liquid Chromatography (HPLC) method. Isoflavone extraction process was done using a mixture of methanol and chloroform solutions. Isoflavone extracts obtained will be analysed using High Performance Liquid Chromatography (HPLC) method to determine the content of genistein. The optimum isoflavone extract was obtained from chloroform:methanol (10:1 v/v) solvent ratio. Where as the highest contents of genistein from the 4 th day “rotten tempe” as 26.199 ± 25.146 (mg/g).