文章基本信息

标题：Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran
其他标题：Molecular Characterization of Animal Fasciola spp. Isolates from Kermanshah, Western Iran
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作者：Arezoo BOZORGOMID ; Naser NAZARI ; Hamzeh RAHIMI 等
期刊名称：Iranian Journal of Public Health
印刷版ISSN：2251-6085
电子版ISSN：2251-6093
出版年度：2016
卷号：45
期号：10
页码：1315-1321
语种：English
出版社：Tehran University of Medical Sciences
摘要：Background: We evaluated the genetic diversity of samples identiﬁed morphologically as Fasciola spp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.Methods: We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics of Fasciola species obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species of Fasciola were confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.Results: In Kermanshah, F. hepatica was present in 96% of the samples, F. gigantica was found only in two cattle sample. No hybrid forms were detected in the present study. Conclusion: Our results contribute to clarify the dark spots of Fasciola genotyping in different parts of Iran. Keywords: Fasciola hepatica, Fasciola gigantica, Genotype, PCR, Iran
关键词：Fasciola hepatica; Fasciola gigantica; Genotype; PCR; Iran