文章基本信息

标题：Studies on the Interactions between Pto and Pti1s Proteins from Maize by a Yeast Two-Hybrid System
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作者：Yong Zhu ; Xiao-yan Sun ; Beng Wang 等
期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2012
卷号：4
期号：6
页码：389-392
出版社：MAXWELL Science Publication
摘要：To check whether the protein ZmPto can interact with the protein ZmPti1s, in this study, the gene sequences of ZmPto and ZmPti1a, ZmPti1b were inserted into yeast expression vectors pEG202 and pJG4-5, respectively. Yeast two-hybrid was then conducted to verify their interactions. Results showed that yeast strains neither carrying empty plasmid nor carrying only one single fusion sequence of ZmPti1 or ZmPto can activate reporter genes. Only the yeast strains carrying both fusion sequence of ZmPti1 and ZmPto can activate reporter genes indicating that ZmPto can interact directly with ZmPti1 in the yeast two-hybrid system.
关键词：Maize ; Yeast two-hybrid system ; ZmPto; ZmPti1 ; ; ;