期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2015
卷号：9
期号：9
页码：701-705
DOI：10.19026/ajfst.9.1763
出版社：MAXWELL Science Publication
摘要：Valsa mali var. mali is a causal agent of apple tree canker disease, that causes serious economic losses in China. The lack of understanding of the pathogenic genes and mechanisms of V. mali , which poses difficulties to control the disease. Whereas, previous studies have indicated that xylanase produced by V. mali is a key pathogenic factor. In this study, Xylanase gene was cloned and obtained recombinant expression vector pET- Xylanase , in addition, a prokaryotic expression system for Xylanase protein was established and the expression conditions were optimized. Xylanase protein expressed in Escherichia coli reached a maximum level after induction with 0.5 mmol/L isopropyl &beta-thiogalactopyranoside (IPTG) for 12 h at 30°C. The recombinant protein was confirmed by Western blot analysis using Anti-His mouse monoclonal antibody and the result showed that the induced ~63 kDa band in E. coli was Xylanase protein. The results presented will help to better reveal the xylanase gene functions in the pathogenicity of V. mali .
Loading...
