期刊名称：Advance Journal of Food Science and Technology
印刷版ISSN：2042-4868
电子版ISSN：2042-4876
出版年度：2015
卷号：7
期号：9
页码：685-690
DOI：10.19026/ajfst.7.1628
出版社：MAXWELL Science Publication
摘要：Exopolysaccharide (EPS) produced from lactic acid bacteria has many bioactivities and the role of prebiotic as reported. However, being a well-knowen probiotic, there is little report about EPS-producing bifidobacteria. In this study, several bifidobacteria strains were isolated from baby feces with mupirocin lithium salt improved medium and evaluated for the production of EPS. Culturing strains with MRS medium containing 2% sucrose, one EPS-producing bifidobacteria strain A19 was screened by ethanol precipitation, dialysis, phenol-sulfuric acid method and other methods. By the physiological and biochemical characteristics, 16S rDNA and tuf gene sequence analysis, strain A19 was identified as Bifidobacterium pseudocatenulatum . It was a novel EPS-producing bifidobacteria and the EPS yield was 55.546 mg/L in MRS broth with 2% sucrose. It could be a potential available strain for microecologic products and food starters.
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