文章基本信息

标题：PrimPol is required for replication reinitiation after mtDNA damage
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作者：Rubén Torregrosa-Muñumer ; Josefin M. E. Forslund ; Steffi Goffart 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：43
页码：11398-11403
DOI：10.1073/pnas.1705367114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Eukaryotic PrimPol is a recently discovered DNA-dependent DNA primase and translesion synthesis DNA polymerase found in the nucleus and mitochondria. Although PrimPol has been shown to be required for repriming of stalled replication forks in the nucleus, its role in mitochondria has remained unresolved. Here we demonstrate in vivo and in vitro that PrimPol can reinitiate stalled mtDNA replication and can prime mtDNA replication from nonconventional origins. Our results not only help in the understanding of how mitochondria cope with replicative stress but can also explain some controversial features of the lagging-strand replication.
关键词：DNA repair ; fork rescue ; mtDNA damage ; mtDNA replication