文章基本信息

标题：Effect of cell cycle arrest on intermediate metabolism in the marine diatom Phaeodactylum tricornutum
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作者：Joomi Kim ; Christopher M. Brown ; Min Kyung Kim 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：2017
卷号：114
期号：38
页码：E8007-E8016
DOI：10.1073/pnas.1711642114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The inhibitor NU 2058 [6-(cyclohexylmethoxy)-9 H -purin-2-amine] leads to G1-phase cell cycle arrest in the marine diatom, Phaeodactylum tricornutum, by binding to two cyclin-dependent kinases, CDKA1 and CDKA2. NU 2058 has no effect on photosynthetic attributes, such as F_v/F_m, chlorophyll a /cell, levels of D2 PSII subunits, or RbcL; however, cell cycle arrest leads to unbalanced growth whereby photosynthetic products that can no longer be used for cell division are redirected toward carbohydrates and triacylglycerols (TAGs). Arrested cells up-regulate most genes involved in fatty acid synthesis, including acetyl-CoA carboxylase, and three out of five putative type II diglyceride acyltransferases (DGATs), the enzymes that catalyze TAG production. Correlation of transcriptomes in arrested cells with a flux balance model for P. tricornutum predicts that reactions in the mitochondrion that supply glycerate may support TAG synthesis. Our results reveal that sources of intermediate metabolites and macromolecular sinks are tightly coupled to the cell cycle in a marine diatom, and that arresting cells in the G1 phase leads to remodeling of intermediate metabolism and unbalanced growth.
关键词：cell cycle ; diatom ; intermediate metabolism ; unbalanced growth ; flux balance analysis