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  • 标题:Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of Proteus mirabilis from SouthWest Nigeria
  • 本地全文:下载
  • 作者:Olumuyiwa Samuel Alabi ; Nuno Mendonça ; Olufemi Ezekiel Adeleke
  • 期刊名称:African Health Sciences
  • 印刷版ISSN:1680-6905
  • 电子版ISSN:1729-0503
  • 出版年度:2017
  • 卷号:17
  • 期号:2
  • 页码:356-365
  • DOI:10.4314/ahs.v17i2.9
  • 出版社:Makerere University Medical School(Uganda)
  • 摘要:

    Background: Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported.

    Objectives: This study screened for presence of transferable resistance genes and mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis .

    Methods: A total of 108 P. mirabilis strains collected from five tertiary hospitals in SouthWest Nigeria were subjected to antibiotic susceptibility study using disc-diffusion method. Transferable resistance genes and MGEs were amplified using Polymerase chain reaction (PCR) analysis and amplicons sequenced.

    Results: Varied resistance was observed against all the antibiotics tested. About 56% of the isolates were MDR including those from 0-12 years old children. PCR analysis revealed the presence of aac(6’) -Ib (33.3%), plasmid mediated quinolone resistance (PMQR) genes [qnrA (36.7%), acc(6’) -Ib-cr (5%)], TEM (48.3%), CTX-M (6.7%) and integrons class 1 (58.3%) and class 2 (26.7%). Sequencing analysis revealed bla TEM-1, bla CTX-M-15 associated with ISE cp1 and eight different arrays of gene cassettes: aadA1 , aadA1-qacH , aadB-aadA2 , aadA5 , dfrA7 , dfrA15 , dfrA17 , dfrA17-aadA5 .

    Conclusion: Transferable resistance genes in association with MGEs are present in Nigerian P. mirabilis thus their potential in disseminating resistance.

  • 关键词:Multidrug resistance; resistance determinants; integrase; gene cassettes; Proteus mirabilis
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