文章基本信息

标题：Novel Liquid Chromatographic Method for the Simultaneous Evaluation of Erythromycin and Azithromycin in Human Urine
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作者：Mahmoudi A. ; Boukhechem M.S.
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2017
卷号：8
期号：6
页码：1953-1959
出版社：University of Mohammed Premier Oujda
摘要：A simple new method for the quantitative determination of erythromycin (ERY) and azithromycin (AZI) concentrations in spiked human urine by high-performance liquid chromatography (HPLC) was developed. Separation was performed by using an end-capped ODB RP18 column as a stationary phase and mobile phase consisting of acetonitrile –2-methyl-2-propanol–hydrogenphosphate buffer, pH 6.5, with 1.5% triethylamine (33:7: up to 100, v/v/v), delivered at a flow-rate of 1.0 mL min-1. UV detection is performed at 215 nm for erythromycin and 210 nm for azithromycin. The procedure was quantitatively characterized and, results of selectivity, linearity, detection and quantification limits, precision and accuracy were very satisfactory. The suitability of the method for simultaneous evaluation of the both macrolides was demonstrated by the analysis of spiked samples of human urine. Recovery experiments revealed recovery of 96.54–112.07%.
关键词：Organo;bentonite; Macrolides; erythromycin; azithromycin; human urine; simultaneous determination; HPLC–UV.