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标题：Arbutus unedo L aqueous extract is associated with in vitro and in vivo antioxidant activity
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作者：Mrabti H. N. ; Marmouzi I. ; Sayah K. 等
期刊名称：Journal of Materials and Environmental Science
印刷版ISSN：2028-2508
出版年度：2017
卷号：8
期号：1
页码：217-224
出版社：University of Mohammed Premier Oujda
摘要：Arbutus unedo L. (Ericaceae) roots and leaves have been used traditionally for multiple uses, such asgastrointestinal disorders, skin diseases and urinary infections. Some studies showed an in vitro antioxidantactivity of this plant.Aim of the study:to characterize the nutritional composition and to determine the invivo antioxidant effect of Arbutus unedo L aqueous extract.We used an inductively coupled plasma atomicemission spectroscopy to determine the mineral composition of Arbutus unedo L aqueous extract. The invitro antioxidant activity was measured by calculating the radical-scavenging activity (RSA) as a percentageof 2,2’-Diphenyl-1-picrylhydrazyl hydrate (DPPH) discoloration. The in vivo antioxidant activity wasdetermined using the dosage of malondialdehyde (MDA) and superoxide dismutase (SOD) stress markers indiabetic mice. Phytochemical screening revealed the presence of multiples chemical metabolites suchastannins, anthraquinones and flavonoids. These substances were present with High concentration in leavesand roots.While terpenoids were more present in leaves than in roots. Saponins and alkaloids are totalyabsent for both of them. Moreover, mineral analysis demonstrated a higher content of Na and Zn in roots,and interesting amounts of P, Mg, Ca, Mn, Fe and K in leaves. The aqueous extracts of both parts exhibitedan antioxidant power with IC50 of 4.52±0.69 and 7.24±0.73µg/mL in DPPH assay, in root sand leavesrespectively. There was no significant difference between Arbutus unedo L group and methformin group(p> 0.05) in all of the examined parameters(MDA and SOD) in liver and kidney tissues. In liver and kidney,the levels of MDA in Arbutus unedo L group (p < 0.05) and methformin group (p <0.05) were significantlylesser thanin control group. Inversely, the two study groups presented a high level of SOD compared tocontrol group. Our results show that aqueous extract of Arbtus unedo L is associated with an in vivoantioxidant activity and a lack of certain toxic substances in its composition. Other investigations arenecessary to determine the importance of this antioxidant effect in animals and in humans.
关键词：Arbutus unedo L.; Phenolic compounds; Mineral; Antioxidant; MDA; SOD