文章基本信息

标题：Universal conservation in translation initiation revealed by human and archaeal homologs of bacterial translation initiation factor IF2
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作者：Joon H. Lee ; Sang Ki Choi ; Antonina Roll-Mecak 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：8
页码：4342-4347
DOI：10.1073/pnas.96.8.4342
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Binding of initiator methionyl-tRNA to ribosomes is catalyzed in prokaryotes by initiation factor (IF) IF2 and in eukaryotes by eIF2. The discovery of both IF2 and eIF2 homologs in yeast and archaea suggested that these microbes possess an evolutionarily intermediate protein synthesis apparatus. We describe the identification of a human IF2 homolog, and we demonstrate by using in vivo and in vitro assays that human IF2 functions as a translation factor. In addition, we show that archaea IF2 can substitute for its yeast homolog both in vivo and in vitro. We propose a universally conserved function for IF2 in facilitating the proper binding of initiator methionyl-tRNA to the ribosomal P site.
关键词：eukaryotic translation initiation factor ; FUN12 ; initiator tRNA