文章基本信息

标题：p53-induced DNA bending and twisting: p53 tetramer binds on the outer side of a DNA loop and increases DNA twisting
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作者：Akhilesh K. Nagaich ; Victor B. Zhurkin ; Stewart R. Durell 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：5
页码：1875-1880
DOI：10.1073/pnas.96.5.1875
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：DNA binding activity of p53 is crucial for its tumor suppressor function. Our recent studies have shown that four molecules of the DNA binding domain of human p53 (p53DBD) bind the response elements with high cooperativity and bend the DNA. By using A-tract phasing experiments, we find significant differences between the bending and twisting of DNA by p53DBD and by full-length human wild-type (wt) p53. Our data show that four subunits of p53DBD bend the DNA by 32-36{degrees
关键词：wild-type p53 ; p53 DNA binding domain ; DNA–protein complex ; A-tract phasing analysis ; bent DNA