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标题：Identification of an early T cell progenitor for a pathway of T cell maturation in the bone marrow
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作者：Sussan Dejbakhsh-Jones ; Samuel Strober
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：25
页码：14493-14498
DOI：10.1073/pnas.96.25.14493
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We have identified a rare ({approx}0.05-0.1%) population of cells (Thy-1hiCD16+CD44hiCD2-TCR{beta}-B220-Mac-1-NK1.1-) in the adult mouse bone marrow that generates CD4+ and CD8+ TCR{beta}+ T cells after tissue culture for 48 hr in the presence of Ly5 congenic marrow cells. The essential stages in the maturation of the progenitors were determined; the stages included an early transition from CD2-CD16+CD44hiTCR{beta}- to CD2+CD16int/-CD44int/-TCR{beta}- cells, and a later transition to CD4+CD8+TCR{beta}+ double-positive T cells that rapidly generate the CD4+ and CD8+ single-positive T cells. The maturation of the progenitors is almost completely arrested at the CD2+TCR{beta}- stage by the presence of mature T cells at the initiation of cultures. This alternate pathway is supported by the marrow microenvironment; it recapitulates critical intermediary steps in intrathymic T cell maturation.