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标题：Mass spectrometric determination of dioxygen bond splitting in the “peroxy” intermediate of cytochrome c oxidase
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作者：Marian Fabian ; William W. Wong ; Robert B. Gennis 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：23
页码：13114-13117
DOI：10.1073/pnas.96.23.13114
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The "peroxy" intermediate (P form) of bovine cytochrome c oxidase was prepared by reaction of the two-electron reduced mixed-valence CO complex with 18O2 after photolytic removal of CO. The water present in the reaction mixture was recovered and analyzed for 18O enrichment by mass spectrometry. It was found that approximately one oxygen atom (18O) per one equivalent of the P form was present in the bulk water. The data show that the oxygen-oxygen dioxygen bond is already broken in the P intermediate and that one oxygen atom can be readily released or exchanged with the oxygen of the solvent water.