文章基本信息

标题：Single-copy transgenic wheat generated through the resolution of complex integration patterns
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作者：Vibha Srivastava ; Olin D. Anderson ; David W. Ow 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：20
页码：11117-11121
DOI：10.1073/pnas.96.20.11117
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Genetic transformation of plants often results in multiple copies of the introduced DNA at a single locus. To ensure that only a single copy of a foreign gene resides in the plant genome, we used a strategy based on site-specific recombination. The transformation vector consists of a transgene flanked by recombination sites in an inverted orientation. Regardless of the number of copies integrated between the outermost transgenes, recombination between the outermost sites resolves the integrated molecules into a single copy. An example of this strategy has been demonstrated with wheat transformation, where four of four multiple-copy loci were resolved successfully into single-copy transgenes.
关键词：wheat transformation ; site-specific recombination ; Cre- lox