文章基本信息

标题：An inverse relationship between T cell receptor affinity and antigen dose during CD4+ T cell responses in vivo and in vitro
本地全文：下载
作者：William Rees ; Jeremy Bender ; T. Kent Teague 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：17
页码：9781-9786
DOI：10.1073/pnas.96.17.9781
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas. A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.