文章基本信息

标题：The lack of consensus for I-Ag7-peptide binding motifs: Is there a requirement for anchor amino acid side chains?
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作者：Eugenio Carrasco-Marin ; Osami Kanagawa ; Emil R. Unanue 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：15
页码：8621-8626
DOI：10.1073/pnas.96.15.8621
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We discuss here the problems in identifying sequence motifs of peptides that bind to I-Ag7, the class II histocompatibility molecule of NOD diabetic mice. We present studies that indicate a minor contribution of amino acid side chains for binding. A peptide from the E chain binds to I-Ag7 molecules and is recognized by CD4 T cells. By producing single-residue mutations we identified four residues that were considered to contact the T cell receptor. No residue was found to be essential for binding to I-Ag7: a peptide that contained the T cell contact residues, on a backbone of alanines, bound to I-Ag7 and stimulated the T cells. We conclude that peptides can bind to I-Ag7 without the requirement for residues with prominent side chains to anchor them.