文章基本信息

标题：Divergent evolution of membrane protein topology: The Escherichia coli RnfA and RnfE homologues
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作者：Annika Sääf ; Marie Johansson ; Erik Wallin 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1999
卷号：96
期号：15
页码：8540-8544
DOI：10.1073/pnas.96.15.8540
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Although the molecular evolution of protein tertiary structure and enzymatic activity has been studied for decades, little attention has been paid to the evolution of membrane protein topology. Here, we show that two closely related polytopic inner membrane proteins from Escherichia coli have evolved opposite orientations in the membrane, which apparently has been achieved by the selective redistribution of positively charged amino acids between the polar segments flanking the transmembrane stretches. This example of divergent evolution of membrane protein topology suggests that a complete inversion of membrane topology is possible with relatively few mutational changes even for proteins with multiple transmembrane segments.