文章基本信息

标题：Inhibition of FtsZ polymerization by SulA, an inhibitor of septation in Escherichia coli
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作者：Amit Mukherjee ; Chune Cao ; Joe Lutkenhaus 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：6
页码：2885-2890
DOI：10.1073/pnas.95.6.2885
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The bacterial cell division protein FtsZ assembles into the cytokinetic Z ring that directs cytokinesis in prokaryotes. In Escherichia coli the formation of the Z ring is prevented by induction of the cell division inhibitor SulA (SfiA), a component of the SOS response. Here we show that a MalE-SulA fusion that retains this inhibitory function in vivo inhibits the GTPase activity and polymerization of FtsZ in vitro. MalE-SulA10, which does not block Z ring formation in vivo, is unable to inhibit the GTPase activity and polymerization in vitro. Furthermore, FtsZ114, which is refractory to SulA in vivo, is not inhibited by MalE-SulA. These results indicate that SulA blocks Z ring formation by blocking FtsZ polymerization.
关键词：cytokinesis ; GTPase ; tubulin ; polymerization