文章基本信息

标题：Bcl-2 prevents apoptotic mitochondrial dysfunction by regulating proton flux
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作者：Shigeomi Shimizu ; Yutaka Eguchi ; Wataru Kamiike 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：4
页码：1455-1459
DOI：10.1073/pnas.95.4.1455
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：We and others have recently shown that loss of the mitochondrial membrane potential ({Delta}{psi}) precedes apoptosis and chemical-hypoxia-induced necrosis and is prevented by Bcl-2. In this report, we examine the biochemical mechanism used by Bcl-2 to prevent {Delta}{psi} loss, as determined with mitochondria isolated from a cell line overexpressing human Bcl-2 or from livers of Bcl-2 transgenic mice. Although Bcl-2 had no effect on the respiration rate of isolated mitochondria, it prevented both {Delta}{psi} loss and the permeability transition (PT) induced by various reagents, including Ca2+, H2O2, and tert-butyl hydroperoxide. Even under conditions that did not allow PT, Bcl-2 maintained {Delta}{psi