文章基本信息

标题：Mammalian DNA topoisomerase IIIα is essential in early embryogenesis
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作者：Wei Li ; James C. Wang
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：3
页码：1010-1013
DOI：10.1073/pnas.95.3.1010
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Targeted disruption of the mouse TOP3 gene encoding DNA topoisomerase III was carried out to study the physiological functions of the mammalian type IA DNA topoisomerase. Whereas heterozygous top3+/- mutant mice were found to resemble phenotypically their TOP3+/+ litermates, no viable top3-/- homozygotes were found among over 100 progeny of top3+/- intercrosses. Examination of embryos dissected from decidual swellings and in vitro culturing of blastocysts from top3+/- intercrosses showed that implantation of top3-/- embryos and the induction of decidualization could occur, but viability of these embryos was severely compromised at an early stage of development. The requirement of mouse DNA topoisomerase III during early embryogenesis is discussed in terms of its plausible role in chromosome replication and its interaction with the RecQ/SGS1 family of DNA helicases, whose members include the Bloom's syndrome and the Werner's syndrome gene products.
关键词：targeted gene disruption ; Δ top3α mice ; genome instability