文章基本信息

标题：In situ synthesis of β-glucan microfibrils on tobacco plasma membrane sheets
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作者：Noriko Hirai ; Seiji Sonobe ; Takahisa Hayashi 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：25
页码：15102-15106
DOI：10.1073/pnas.95.25.15102
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：A major concern in plant morphogenesis is whether cortical microtubules are responsible for the arrangement and action of {beta}-glucan synthases in the plasma membrane. We prepared isolated plasma membrane sheets with cortical microtubules attached and tested whether {beta}-glucan synthases penetrated through the membrane to form microfibrils and whether these synthases moved in the fluid membrane along the cortical microtubules. This technique enabled us to examine synthesis of {beta}-glucan as a fiber with a two-dimensional structure. The synthesis of {beta}-glucan microfibrils was directed in arrays by cortical microtubules at many loci on the membrane sheets. The microfibrils were mainly arranged along the microtubules, but the distribution of microfibrils was not always parallel to that of the microtubules. The rate of {beta}-glucan elongation as determined directly on the exoplasmic surface was 620 nm per min. When the assembly of microtubules was disrupted by treatment with propyzamide, the {beta}-glucans were not deposited in arrays but in masses. This finding shows that the arrayed cortical microtubules are not required for {beta}-glucan synthesis but are required for the formation of arranged microfibrils on the membrane sheet.
关键词：cortical microtubule/UDP-glucose/taxol