文章基本信息

标题：Transcriptional repression by AML1 and LEF-1 is mediated by the TLE/Groucho corepressors
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作者：Ditsa Levanon ; Robert E. Goldstein ; Yael Bernstein 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：20
页码：11590-11595
DOI：10.1073/pnas.95.20.11590
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：The mammalian AML/CBF runt domain (RD) transcription factors regulate hematopoiesis and osteoblast differentiation. Like their Drosophila counterparts, most mammalian RD proteins terminate in a common pentapeptide, VWRPY, which serves to recruit the corepressor Groucho (Gro). Using a yeast two-hybrid assay, in vitro association and pull-down experiments, we demonstrate that Gro and its mammalian homolog TLE1 specifically interact with AML1 and AML2. In addition to the VWRPY motif, other C-terminal sequences are required for these interactions with Gro/TLE1. TLE1 inhibits AML1-dependent transactivation of the T cell receptor (TCR) enhancers and {beta