文章基本信息

标题：Chromosomal transposition of a Tc1/mariner-like element in mouse embryonic stem cells
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作者：Guangbin Luo ; Zoltán Ivics ; Zsuzsanna Izsvák 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：18
页码：10769-10773
DOI：10.1073/pnas.95.18.10769
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：Mouse has become an increasingly important organism for modeling human diseases and for determining gene function in a mammalian context. Unfortunately, transposon-tagged mutagenesis, one of the most valuable tools for functional genomics, still is not available in this organism. On the other hand, it has long been speculated that members of the Tc1/mariner-like elements may be less dependent on host factors and, hence, can be introduced into heterologous organisms. However, this prediction has not been realized in mice. We report here the chromosomal transposition of the Sleeping Beauty (SB) element in mouse embryonic stem cells, providing evidence that it can be used as an in vivo mutagen in mice.
关键词：transposon