文章基本信息

标题：Failure of egg cylinder elongation and mesoderm induction in mouse embryos lacking the tumor suppressor smad2
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作者：Michael Weinstein ; Xiao Yang ; Cuiling Li 等
期刊名称：Proceedings of the National Academy of Sciences
印刷版ISSN：0027-8424
电子版ISSN：1091-6490
出版年度：1998
卷号：95
期号：16
页码：9378-9383
DOI：10.1073/pnas.95.16.9378
语种：English
出版社：The National Academy of Sciences of the United States of America
摘要：smad genes constitute a family of nine members whose products serve as intracellular mediators of transforming growth factor {beta} signals. SMAD2, which is a tumor suppressor involved in colorectal and lung cancer, has been shown to induce dorsal mesoderm in Xenopus laevis in response to transforming growth factor {beta} and activins. The smad2 gene is expressed ubiquitously during murine embryogenesis and in many adult mouse tissues. Animals that lacked smad2 died before 8.5 days of development (E8.5). E6.5 homozygous mutants were smaller than controls, lacked the extraembryonic portion of the egg cylinder, and appeared strikingly similar to E6.5 smad4 mutants. This similarity was no longer evident at E7.5, however, because the smad2 mutants contained embryonic ectoderm within their interiors. Molecular analysis showed that smad2 mutant embryos did not undergo gastrulation or make mesoderm. The results demonstrate that smad2 is required for egg cylinder elongation, gastrulation, and mesoderm induction.
关键词：transforming growth factor β/ smad4 /gastrulation/extraembryonic membranes